Description
Description: Recombinant full-length human GSK3beta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Scientific Background: GSK3beta is a serine threonine protein kinase that was originally identified as the kinase that phosphorylate and inhibits glycogen synthase (1). GSK3beta is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3 beta into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies (2)